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10X Genomics permeabilization enzyme pn2000214
Permeabilization Enzyme Pn2000214, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+enzyme/permeabilization+enzyme/pm40185750-528-3-5
Average 90 stars, based on 1 article reviews
permeabilization enzyme pn2000214 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Incubation:

Article Title: Spatial transcriptomics of the aging mouse brain reveals origins of inflammation in the white matter.
Article Snippet: The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. .. The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. ..

Article Title: Spatial transcriptomics of the aging mouse brain reveals origins of inflammation in the white matter
Article Snippet: To optimize permeabilization time for mouse brain tissue, the Visium spatial tissue optimization slide was placed in the slide cassette (10X Genomics, PN3000406). .. The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3 min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. ..

Gene Expression:

Article Title: Spatial transcriptomics of the aging mouse brain reveals origins of inflammation in the white matter.
Article Snippet: The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. .. The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. ..

Article Title: Spatial transcriptomics of the aging mouse brain reveals origins of inflammation in the white matter
Article Snippet: To optimize permeabilization time for mouse brain tissue, the Visium spatial tissue optimization slide was placed in the slide cassette (10X Genomics, PN3000406). .. The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3 min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. ..

Article Title: Spatial transcriptomics analysis of neoadjuvant cabozantinib and nivolumab in advanced hepatocellular carcinoma identifies independent mechanisms of resistance and recurrence
Article Snippet: The fixed samples were stained with hematoxylin and eosin (H&E) and imaged using the Nanozoomer scanner (Hamamatsu) at 40x magnification. .. Samples were permeabilized for 30 minutes at 37°C with the Permeabilization Enzyme provided with the Visium Spatial Gene Expression Reagent Kits (10x Genomics). ..

Imaging:

Article Title: Spatio-temporal transcriptomics of chromothriptic SHH-medulloblastoma identifies multiple genetic clones that resist treatment and drive relapse
Article Snippet: .. Permeabilization was performed on a pre-heated thermal cycler at 37 °C with Permeabilization Enzyme for 12 min. Permeabilization time was determined in the previous Tissue Optimization Experiment following the Visium Spatial User Guide CG000238_VisiumSpatialTissueOptimizationUserGuide_RevC using the Visium Spatial Tissue Optimization Slide & Reagents Kit (#1000193, 10x Genomics), by permeabilizing tissue for different times, synthesising fluorescent cDNA, imaging, and determining the optimal signal. ..

Saline:

Article Title: Spatial and single-cell transcriptomics decipher the cellular environment containing HLA-G+ cancer cells and SPP1+ macrophages in colorectal cancer.
Article Snippet: .. To optimize the permeabilization time of the sections, they were treated with the permeabilization enzyme (10x Genomics: 2000214) for 60 min, washed in 0.13 saline sodium citrate (SSC) buffer (Sigma-Aldrich), and imaged. .. Tissues were permeabilized with the permeabilization enzyme (10x Genomics: 2000214) for 50 min, washed with 0.13 SSC buffer (Sigma-Aldrich), and imaged.



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